Paper Details
Fundamental Toxicological Sciences
Vol. 1
No. 4
December 24, 2014
p.161-164
Toxicomics Report
CDC23 knockdown reinforces methylmercury sensitivity in HEK293 cells
- Gi-Wook Hwang (Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University / gwhwang@m.tohoku.ac.jp)
Ke Du
1)
2)
,
Tsutomu Takahashi
1)
,
Miyuki Iwai-Shimada
1)
,
Nobuhiko Miura
3)
,
Akira Naganuma
1)
,
Gi-Wook Hwang
1)
1) Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University , 2) Department of Pharmacology, China Medical University , 3) Division of Health Effects Research, Japan National Institute of Occupational Safety and Health
1) Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University , 2) Department of Pharmacology, China Medical University , 3) Division of Health Effects Research, Japan National Institute of Occupational Safety and Health
Keywords: Methylmercury, CDC23, Ubiquitin ligase
Abstracts
The ubiquitin-proteasome system is believed to play an important role in the determination of cell sensitivity to methylmercury. The ubiquitin ligase enzyme is involved in the recognition of substrate proteins that are degraded by the ubiquitin-proteasome system. In this study, the ubiquitin ligase species affecting methylmercury sensitivity was investigated by the gene interference method. We found that the inhibition of expression of the gene for Cell division cycle 23 (CDC23), a constitutional component of the ubiquitin ligase anaphase promoting complex/cyclosome, sensitized HEK 293 cells to methylmercury.