Paper Details
Fundamental Toxicological Sciences
Vol. 9
No. 5
October 07, 2022
p.159-162
Toxicomics Report
Deubiquitinase USP54 attenuates methylmercury toxicity in human embryonic kidney 293 cells
- Gi-Wook Hwang (Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University / Laboratory of Environmental and Health Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University / hwang@tohoku-mpu.ac.jp)
Jin-Yong Lee
1)
2)
,
Jong-Mu Kim
1)
,
Takuya Noguchi
3)
,
Atsushi Matsuzawa
3)
,
Akira Naganuma
1)
,
Gi-Wook Hwang
1)
4)
1) Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University , 2) Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University , 3) Laboratory of Health Chemistry, Graduate School of Pharmaceutical Sciences, Tohoku University , 4) Laboratory of Environmental and Health Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University
1) Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University , 2) Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University , 3) Laboratory of Health Chemistry, Graduate School of Pharmaceutical Sciences, Tohoku University , 4) Laboratory of Environmental and Health Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University
Keywords: Methylmercury, Deubiquitinase, USP54, RNA interference
Abstracts
Deubiquitinases are an important regulator of ubiquitin-mediated signaling pathways because of their ability to cleave the isopeptide bond that connects the ubiquitin chain to the target protein. We previously reported that the expression of many proteins involved in methylmercury toxicity is regulated by the ubiquitin-proteasome system. In this study, double-stranded siRNAs against mRNAs of approximately 60 deubiquitinases were transfected into human embryonic kidney (HEK) 293 cells, and ubiquitin-specific protease 54 (USP54) was identified as a deubiquitinase that increases the sensitivity of HEK293 cells to methylmercury by RNA interference.