Fundamental Toxicological Sciences

Paper Details

Fundamental Toxicological Sciences
Vol. 2 No. 4 August 25, 2015 p.137-145
Original Article
Effects of cisplatin on testicular enzymes and Sertoli cell function in rats
  • Yingbiao Sun (Department of Toxicology, School of Public Health, Lanzhou University, Lanzhou, China /
Zhifei Liu , Yingbiao Sun , Li Su , Yifan Sun , Shibo Kong , Xuhong Chang , Fang Guo , Wei Li , Junjie Guo , Jin Li
Department of Toxicology, School of Public Health, Lanzhou University, Lanzhou, China
Keywords: Cisplatin, Testicular enzymes, MT-1, HO-1, ABP, Inhibin╬▓-B

Cisplatin (CP) is one of important tumour chemotherapeutic agents in humans. Previous reports claim that CP can cause testicular toxicity. The aim of this study was to evaluate the potential effects of CP in the testes of rats. Male Wistar rats were intraperitoneally administered CP at 1.0, 2.5, and 5.0 mg/kg for three consecutive days. After exposure, CP significantly inhibited the testicular activities of succinate dehydrogenase (SDH) and malate dehydrogenase (MDH), but it significantly elevated the activities of acid phosphatase (ACP), alkaline phosphatase (AKP), and lactate dehydrogenase (LDH) in the 5.0 mg/kg group. The decreased levels of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and metallothionein-1 (MT-1) mRNA as well as the increased levels of malondialdehyde (MDA) and haemoxygenase-1 (HO-1) mRNA showed that CP could increase oxidative stress in rat testes. Western blot analysis showed that the levels of transferrin, vimentin, androgen binding protein (ABP) and inhibin╬▓-B decreased significantly in the CP 2.5 and 5.0 mg/kg groups compared with the control group. These findings indicated that the inhibited enzymes, oxidative stress, and the down-regulation of Sertoli cell function-related proteins play pivotal roles in CP-induced testicular damage.