- Akira Naganuma (Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University / email@example.com)
1) Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University , 2) Department of Microbiology, Tohoku Pharmaceutical University
HEK293 cells were cultured in medium containing methylmercury (MeHg), followed by replacement with MeHg-free medium and further culturing. Thus, MeHg preconditioning medium (MeHg-PM) were obtained. Untreated HEK293 cells and C17.2 cells (mouse neural stem cells) were placed in the obtained MeHg-PM for culturing, which resulted in significantly inhibited cell growth. This cell growth inhibition was not affected by heating or proteinase K treatment, suggesting that neither proteins nor peptides caused the growth inhibition.