Fundamental Toxicological Sciences

Paper Details

Fundamental Toxicological Sciences
Vol. 4 No. 1 February 24, 2017 p.31-35
Toxicomics Report
Proteomic analysis of valproic acid-induced embryotoxicity in cultured post-implantation rat embryos
  • Makoto Usami (Division of Pharmacology, National Institute of Health Sciences /
Makoto Usami 1) , Mina Takamatsu 2) , Shugo Kazama 2) , Katsuyoshi Mitsunaga 3) , Atsuko Miyajima 4) , Tomohiko Irie 1) , Osamu Doi 5) , Tatsuya Takizawa 2) , Teruko Nagi 1) , Momoko Sunouchi 1)
1) Division of Pharmacology, National Institute of Health Sciences , 2) School of Veterinary Medicine, Azabu University , 3) School of Pharmaceutical Sciences, Toho University , 4) Division of Medical Devices, National Institute of Health Sciences , 5) Laboratory of Animal Reproduction, United Graduate School of Agricultural Science, Gifu University
Keywords: Valproic acid, Embryotoxicity, Proteomics, Rat

Protein expression changes were examined in day 10.5 rat embryos cultured for 24 hr in the presence of valproic acid (VPA), using two-dimensional electrophoresis and mass spectrometry. Exposure to VPA at an embryotoxic concentration of 1.2 mM resulted in quantitative changes in many embryonic protein spots (22 decreased and 29 increased). For the increased protein spots, 10 proteins were identified, including alpha-fetoprotein, phosphorylated cofilin-1, and serum albumin. These proteins are candidate protein biomarkers that may be involved in embryotoxic mechanisms.