Fundamental Toxicological Sciences

Paper Details

Fundamental Toxicological Sciences
Vol. 9 No. 4 September 07, 2022 p.117-122
Letter
Leaf extracts from Camellia sinensis and Argania spinosa suppress oxidative stress and chemokine release in human 3-dimensional cultured epidermis exposed to PM2.5 collected with cyclonic separation
  • Yasuhiro Ishihara (Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University / ishiyasu@hiroshima-u.ac.jp)
Maori Kono 1) 2) , Tomoaki Okuda 3) , Masayuki Takaishi 1) 2) , Hidefumi Ikeda 2) , Nami Ishihara 4) , Yasuhiro Ishihara 4)
1) Laboratory of advanced cosmetic science, Graduate School of Pharmaceutical Sciences, Osaka University , 2) Product Assurance Division, Mandom Corporation , 3) Faculty of Science and Technology, Keio University , 4) Program of Biomedical Science, Graduate School of Integrated Sciences for Life, Hiroshima University
Keywords: Leaf extracts, 3D epidermis culture, Chemokines, PM2.5, Oxidative stress
Abstracts

Skin is the primary tissue exposed to ambient air pollution because it acts as an interface between the body and the surrounding atmosphere. We previously reported that particulate matter 2.5 (PM2.5) induced oxidative stress and subsequent chemokine release in the human epidermis, followed by neutrophil chemotaxis. We identified in this study that the leaf extracts from Camellia sinensis and Argania spinosa showed high radical scavenging activity as evaluated by 2,2-diphenyl-1-(2,4,6-trinitrophenyl)-hydrazinyl and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid assays. PM2.5 exposure induced lipid peroxidation, IL-8 release and neutrophil migration in human 3-dimensional cultured epidermis. Pretreatment with leaf extracts from Camellia sinensis or Argania spinosa significantly suppressed the above harmful effects elicited by PM2.5. Taken together, both extracts can protect the epidermis from PM2.5 exposure. Camellia sinensis and Argania spinosa extracts could be added to a novel cosmetic that protects skin from air pollution.