Fundamental Toxicological Sciences

Paper Details

Fundamental Toxicological Sciences
Vol. 1 No. 1 September 04, 2014 p.15-18
Original Article
Cytotoxic activity and abdominal writhes promoted by snake venom from Philodryas nattereri Steindachner, 1870
  • Marinetes Dantas de Aquino Nery (Department of Biology, Federal University of Ceará, Brazil /
Marinetes Dantas de Aquino Nery 1) , Hermano Damasceno de Aquino 1) , Rayane de Tasso Moreira Ribeiro 2) , Erik de Aquino Nery 3) , Thiala Soares Josino da Silva 4) , Rodrigo Tavares Dantas 4) , Ramon Róseo Paula Pessoa Bezerra de Menezes 4) , Marcus Felipe Bezerra da Costa 4) , Alice Maria Costa Martins 4) , Natacha Teresa Queiroz Alves 4) , Helena Serra Azul Monteiro 4)
1) Department of Biology, Federal University of Ceará, Brazil , 2) Institut of Biosciences, University of São Paulo, Brazil , 3) General Hospital of Fortaleza, Brazil , 4) Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Brazil
Keywords: Snake venom, Citotoxic activity, Cell culture, Abdominal writhes

Philodryas nattereri is distributed in arid and semiarid regions of South America and is most common in northeastern Brazil. The aims of the work were to investigate the effects of the venom from P. nattereri in cultured of MDCK and RAW cells and abdominal writhes in mice. Based on oxidative metabolism, it was possible to observe that the venom was capable of significantly reducing cell viability only at higher concentrations of venom at 50 and 100 μg/mL for MDCK cells, while in 200 μg/mL to RAW cells, with an IC50 of 169.5 μg/mL. Regarding writhing in mice promoted by the poison and acetic acid, it held a greater number of writhes when compared to promoted by saline. The venom of P. nattereri has a cytotoxic effect in MDCK and RAW cells and abdominal writhes, which appears to be similar to those caused by acetic acid.