Paper Details
Fundamental Toxicological Sciences
Vol. 2
No. 5
December 02, 2015
p.223-226
Original Article
Methylmercury induces release of a cytotoxic factor from HEK293 cells into medium
- Akira Naganuma (Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University / naganuma@m.tohoku.ac.jp)
Takashi Toyama
1)
,
Souichi Murakami
1)
,
Shusuke Kuge
2)
,
Gi-Wook Hwang
1)
,
Akira Naganuma
1)
1) Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University , 2) Department of Microbiology, Tohoku Pharmaceutical University
1) Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University , 2) Department of Microbiology, Tohoku Pharmaceutical University
Keywords: Methylmercury, Toxicity, HEK 293 cells, C17.2 cells
Abstracts
HEK293 cells were cultured in medium containing methylmercury (MeHg), followed by replacement with MeHg-free medium and further culturing. Thus, MeHg preconditioning medium (MeHg-PM) were obtained. Untreated HEK293 cells and C17.2 cells (mouse neural stem cells) were placed in the obtained MeHg-PM for culturing, which resulted in significantly inhibited cell growth. This cell growth inhibition was not affected by heating or proteinase K treatment, suggesting that neither proteins nor peptides caused the growth inhibition.