- Hiroki Yoshioka (College of Pharmacy, Kinjo Gakuin University / firstname.lastname@example.org)
- Akito Nagatsu (College of Pharmacy, Kinjo Gakuin University / email@example.com)
1) College of Pharmacy, Kinjo Gakuin University , 2) Division of Health Effects Research, Japan National Institute of Occupational Safety and Health
Acute liver disease may develop due to various causes and occur by different mechanisms. Carbon tetrachloride (CCl4), a well-known hepatic toxicant, was selected as a model of alkylating agents that do not induce glutathione depletion. Our previous study indicated CCl4-induced hepatotoxicity was decreased by pretreatment with the Japanese herbal medicine “Juzen-taiho-to” (JTX), suggesting that prophylaxis with JTX protects mice from CCl4-induced acute hepatic toxicity. In contrast, bromobenzene (BB) is a known glutathione-depleting agent. Although BB-induced hepatotoxicity also promotes lipid peroxidation, the mechanism of hepatic injury is different from that of CCl4. Hence, in this study, we investigated whether pretreatment with JTX ameliorated BB-induced hepatotoxicity. Mice injected with BB showed increased plasma levels of hepatic injury markers (alanine aminotransferase and aspartate aminotransferase) in addition to hepatic lipid peroxidation. Pretreatment with JTX decreased BB-induced plasma levels of hepatic injury markers. BB-induced hepatotoxicity is mainly caused by oxidative stress. JTX pretreatment also decreased BB-induced lipid peroxidation. Our results suggest that JTX has the potential to protect against BB-induced hepatotoxicity and modulate oxidative stress.