Fundamental Toxicological Sciences

Paper Details

Fundamental Toxicological Sciences
Vol. 4 No. 2 April 14, 2017 p.77-83
Toxicomics Report
Urinary proteome profile of renal papillary necrosis in experimental model rats
  • Daisuke Sasaki (Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University / Drug Safety Research Labs, Astellas Pharma Inc. / daisuke.sasaki@astellas.com)
Daisuke Sasaki 1) 2) , Masashi Hiramoto 3) , Masatoshi Yuri 4) , Kenjiro Tsubota 2) , Hikaru Mitori 2) , Akira Moriguchi 2) , Akira Unami 2) , Gi-Wook Hwang 1) , Akira Naganuma 1)
1) Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University , 2) Drug Safety Research Labs, Astellas Pharma Inc. , 3) Translational Science Research Labs, Astellas Pharma Inc. , 4) Bioscience Research Labs, Astellas Pharma Inc.
Keywords: Renal injury, Nephrotoxin, Proteomics, iTRAQ
Abstracts

We aimed to identify novel biomarkers using toxicoproteomics to profile the urinary proteome of renal papillary necrosis (RPN) in experimental model rats. RPN was induced in rats by a single intraperitoneal injection of 2-bromoethylamine hydrobromide (BEA). Urinary proteins from four groups (control group; BEA-treated but no RPN; BEA-treated with RPN but no increase in blood urea nitrogen [BUN]; and BEA-treated with RPN and increase in BUN) were pooled. Relative quantitation of pooled urinary proteins was then performed by two-dimensional liquid chromatography-tandem mass spectrometry with the isobaric labeling technique. We found 77 proteins were changed in RPN model rat urine.