Paper Details
Fundamental Toxicological Sciences
Vol. 2
No. 6
December 21, 2015
p.245-247
Toxicomics Report
Knockdown of Acyl-CoA thioesterase 9 gene expression by siRNA confers resistance to arsenite in HEK293 cells
- Akira Naganuma (Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University / naganuma@m.tohoku.ac.jp)
Tsutomu Takahashi
1)
2)
,
Min-Seok Kim
1)
,
Gi-Wook Hwang
1)
,
Shusuke Kuge
3)
,
Akira Naganuma
1)
1) Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University , 2) Department of Environmental Health, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences , 3) Department of Microbiology, Tohoku Pharmaceutical University
1) Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University , 2) Department of Environmental Health, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences , 3) Department of Microbiology, Tohoku Pharmaceutical University
Keywords: Arsenite, ACOT9, Acyl-Coenzyme A thioesterase
Abstracts
Arsenic is a hazardous element that exists widely in the environment. To elucidate the molecular mechanism involved in the onset of arsenic toxicity, we comprehensively searched for genes affecting the sensitivity of HEK293 cells to arsenite by using a siRNA library targeting approximately 17,000 human genes. As a result, we identified ACOT9, one of the acyl-coenzyme A thioesterases, as the gene conferring arsenite resistance to cells by knockdown.