Fundamental Toxicological Sciences

Paper Details

Fundamental Toxicological Sciences
Vol. 1 No. 3 November 06, 2014 p.95-99
Original Article
A screening method for growth-dependent genes involved in the cytotoxicity of chemicals in Saccharomyces cerevisiae
  • Akira Naganuma (Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University / naganuma@m.tohoku.ac.jp)
JunXuan Zhu 1) , Tsutomu Takahashi 1) , Shusuke Kuge 1) 2) , Gi-Wook Hwang 1) , Akira Naganuma 1)
1) Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University , 2) Department of Microbiology, Tohoku Pharmaceutical University
Keywords: Screening method, Essential genes, Arsenite, Resistance, Yeast
Abstracts

When considering mechanisms of toxicity development to chemical substances, one potentially important mechanism is the selective inhibition of proteins essential for cell growth (target molecules). In this study, to detect the target molecules of chemical substances, we established a method for comprehensively screening for essential proteins that confer resistance against chemical substances via overexpression in yeast. We used budding yeast, a common eukaryotic model organism, to produce yeast strains showing overexpression of different genes encoding essential proteins. This method was used to search for overexpressed genes conferring arsenite resistance in yeast, and as a result, we successfully identified ten types of new genes correlated with arsenite resistance.