Rare sugar D-allulose prevents obesity; however, an excessive and continuous intake of D-allulose may induce weight increases in the liver and kidney without apparent pathological and functional abnormalities. Conversely, there has not been reported about how these parameters will change after cessation of D-allulose intake. In this study, effects of a 10-week D-allulose cessation on liver and kidneys weights and biomarkers were investigated in rats previously fed a D-allulose containing diet for 4 weeks. Wistar rats were fed a control diet (C, n=16) or a 3% D-allulose diet (DA, n=16) for 4 weeks, and then the half of rats in the C and DA subgroups were dissected, while the other half of rats were fed the control diet for 10 weeks (C-C and DA-C, n=8, respectively). At the end of the first 4 weeks period, halves of rats in each diet group were euthanized, and the serum, urine, liver, and kidneys were used for pathological and biochemical analyses. The remaining rats were also similarly treated at the end of latter 10 weeks treatment. At week 4, the relative weights of the liver and kidneys were higher in the DA group than in
the C group, but these differences were disappeared by cessation of D-allulose. No abnormal parameters related to liver and kidneys functions were observed in the serum and urine. These findings suggested that D-allulose-induced increases in the liver and kidneys weights could be recovered to the normal levels by D-allulose cessation without accompanying functional and pathological abnormalities.