Paper Details
Fundamental Toxicological Sciences
Vol. 8
No. 5
October 20, 2021
p.157-160
Toxicomics Report
Knockdown of deubiquitinating enzyme Usp34 confers resistance to methylmercury in HEK293 cells
- Gi-Wook Hwang (Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University / Laboratory of Environmental and Health Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University / hwang@tohoku-mpu.ac.jp)
Jong-Mu Kim
1)
,
Jin-Yong Lee
1)
2)
,
Min-Seok Kim
1)
3)
,
Sawako Shindo
4)
,
Takeshi Kumagai
4)
,
Akira Naganuma
1)
,
Gi-Wook Hwang
1)
4)
1) Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University , 2) Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University , 3) Inhalation Toxicology Research Group, Korea Institute of Toxicology, Korea , 4) Laboratory of Environmental and Health Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University
1) Laboratory of Molecular and Biochemical Toxicology, Graduate School of Pharmaceutical Sciences, Tohoku University , 2) Laboratory of Pharmaceutical Health Sciences, School of Pharmacy, Aichi Gakuin University , 3) Inhalation Toxicology Research Group, Korea Institute of Toxicology, Korea , 4) Laboratory of Environmental and Health Sciences, Faculty of Pharmaceutical Sciences, Tohoku Medical and Pharmaceutical University
Keywords: Methylmercury, Sensitivity, Deubiquitinating enzyme, Ubiquitin-specific protease 34 (Usp34), RNA interference
Abstracts
We have previously reported that the ubiquitin–proteasome system, which is a selective proteolytic system, plays an important role in determining sensitivity to methylmercury in various cultured cells. Deubiquitinating enzyme is a negative regulator of protein degradation through the ubiquitin–proteasome system. In the present study, we searched for deubiquitinating enzymes that affect sensitivity to methylmercury by RNA interference, and identified ubiquitin-specific protease 34 (Usp34) as a deubiquitinating enzyme that confers methylmercury resistance to HEK293 cells by suppressing gene expression.